Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

Alexis C.R. Hoste, Tamara Ruiz, Paloma Fernández‐Pacheco, Miguel Ángel Jiménez‐Clavero, Igor Djadjovski, Sandra Moreno, Alejandro Brun, Thomas A. Edwards, John N. Barr, Paloma Rueda, Patricia Sastre

Rift Valley fever virus (RVFV), Crimean‐Congo haemorrhagic fever virus (CCHFV), Schmallenberg virus (SBV), Bluetongue virus (BTV) and the bacteria complex Mycobacterium tuberculosis (TB) all impact livestock and in some cases also represent a threat to humans. Simultaneous detection of antibodies will reduce the volume of sample needed and the time of analysis. A multiplex assay was developed to RVFV, CCHFV, SBV, BTV and TB. The assays reached high sensitivity (93‐100%) and specificity (96‐99%), and the 5plex assay can be used as a high throughput screening tool to identify regions where emerging pathogens are spreading.

Published August 2020 in Transboundary and Emerging Diseases