Hazara nairovirus requires COPI components in both Arf1-dependent and Arf1- independent stages of its replication cycle
Álvarez-Rodríguez*, J. Fuller*, E. J. A. A. Todd, J. Mankouri, R. Hewson and N. Barr
Hazara nairovirus is classified within the Bunyavirales order, and can be used as a model system for Crimean-Congo hemorrhagic fever virus. Using siRNAs targeting genes involved in cellular trafficking and a recombinant Hazara nairovirus expressing eGFP, two distinct phases in the virus replication cycle were found; an early phase prior to and including protein production that exhibits a minor coat protein 1 (COPI) dependence and Arf1 independence, followed by a second phase involved in infectious virus production that is highly COPI dependent and dependent on Arf1 function. It is intriguing to speculate that the non-canonical method of COPI trafficking shown here may represent a newly-recognised feature of the mammalian cellular transport system.
* contributed equally
Published in Journal of Virology