Viral Metagenomics on Cerebrospinal Fluid

By Arthur W. D. Edridge, Martin Deijs, Ingeborg E. van Zeggeren, Cormac M. Kinsella, Maarten F. Jebbink, Margreet Bakker, Diederik van de Beek, Matthijs C. Brouwer and Lia van der Hoek. 

Identifying the causative pathogen in central nervous system (CNS) infections is crucial for patient management and prognosis. VIDISCA-NGS is one of the available workflows for viral metagenomics, which requires only a small input volume and allows multiplexing of multiple samples per run. The performance was determined on 45 cerebrospinal fluid (CSF) samples containing viruses. The results showed that VIDISCA-NGS has the capacity to detect even some low load RNA viruses yet herpesvirus DNA in clinical samples is probably non-encapsidated and therefore difficult to detect by VIDISCA-NGS. 

Published April 2019 in Genes, in the Special Issue Viral Diagnostics Using Next-Generation Sequencing