Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures

Mitra Gultom, Laura Laloli, and Ronald Dijkman

In this book chapter a revised and improved protocol for the isolation and establishment of well differentiated primary airway epithelial cell cultures from human swine bovine and feline origin and the use of the cultures for the characterization of veterinary coronaviruses. Furthermore, a detailed immunohistochemistry protocol with validated antibodies for the visualization and identification of viral cell tropism is included. The protocols are provided in readily-reproducible step-by step fashion, opening with an introductory overview, a list of the materials and reagents needed, followed by a detailed procedure that is supported with helpful notes offering tips and tricks of the trade as well as troubleshooting advice.

Printed August 2020 in Coronaviruses: Methods and Protocols.